Understanding the effect of temperature on the structural integrity and dynamics of proteins has relevance for various fieldsincluding biotechnology and the maintenance of a stable food supply for the climate emergency. Currently, very few methods are available to probe structural changes in proteins as a function of sub-ambient temperature, and yet many drugs are stored at such temperatures. Here we demonstrate how variable temperature ion mobility-mass spectrometry (VT-IM-MS) can elucidate the role of temperature in conformational landscapes in the form of collision cross sections (CCS) at discrete temperatures.
| Date of Award | 27 Jun 2024 |
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| Original language | English |
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| Awarding Institution | - The University of Manchester
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| Supervisor | Perdita Barran (Main Supervisor) & Nicholas Lockyer (Co Supervisor) |
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- collision cross sections
- ion mobility-mass spectrometry
- conformational landscapes
- intrinsically disordered proteins
Conformational landscapes of rigid and flexible molecules explored with Ion Mobility Mass Spectrometry
Wang, X. (Author). 27 Jun 2024
Student thesis: Phd