Strategies for the design and engineering of enzymes rely almost exclusively on nature's alphabet of twenty canonical amino acids. The range of catalytic mechanisms that can be conceivably designed within enzyme active sites is therefore restricted by the limited number of functional groups presented by the genetic code. The research presented in this thesis describes how genetic code expansion and laboratory evolution can operate in concert to generate enzymes with functions and catalytic mechanisms that are inaccessible using the twenty standard amino acid building blocks.
| Date of Award | 31 Dec 2021 |
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| Original language | English |
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| Awarding Institution | - The University of Manchester
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| Supervisor | Anthony Green (Supervisor) & Sam Hay (Supervisor) |
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Design and Evolution of Enzymes with Non-Canonical Organocatalytic Mechanisms
Burke, A. (Author). 31 Dec 2021
Student thesis: Phd