Engineering Saccharomyces cerevisiae toward n‐butanol production

Abstract

Biobutanol represents a second generation biofuel, which can be producedfrom renewable resources by microorganisms. A Saccharomyces cerevisiae strainbearing the five butanol synthetic genes (hbd, adhe2, crt, ccr and ERG10) wasconstructed, where the hbd, adhe2, crt and ccr genes are derived from Clostridiumbeijerinckii, while ERG10 is a yeast gene. The genes were transformed individually onsingle cassettes, which integrated into specific chromosomal sites. The single integrantstrains were back‐crossed to create a strain bearing all five butanol synthetic genes.The butanol synthetic enzymes appeared to be highly expressed in the cytosol,however, very little butanol was obtained (

Date of Award	1 Aug 2016
Original language	English
Awarding Institution	The University of Manchester
Supervisor	Christopher Grant (Supervisor) & Mark Ashe (Supervisor)