Magnetic Resonance Spectroscopy as part of a comprehensive neuroimaging assessment tool

  • Faezeh Sanaei Nezhad

Student thesis: Phd


Magnetic resonance spectroscopy (MRS) allows the non-invasive measurement of selected biological compounds in vivo. Despite MRS proven potential it is not yet a routine clinical tool operated by clinicians. This is mainly due to the complex procedure of MRS acquisition, lack of standardisation in both acquisition and analysis protocols along with lack of a standard quality control. This thesis intended to address these issues with the focus on four metabolites glutathione, glutamate, glutamine and GABA using MEGA-PRESS pulse sequence. Recommendations on acquisition and spectra analysis is made for the MRS protocol MEGA-PRESS aiming to detect glutathione in vivo. This is based on an investigation of glutathione acquisition in vivo and in vitro and was aimed to answer the question: can glutathione be measured reliably using conventional pulse sequence PRESS or does it require editing? The results showed strong evidence of using editing in order to have a reliable glutathione concentration measurement. An analysis along with a quality control method is also presented to enable the extraction of glutamate and glutamine from a GABA-optimised MEGA-PRESS pulse sequence. This enables simultaneous measurements of GABA, glutamate and glutamine in a single acquisition. A criterion of NAA linewidth
Date of Award1 Aug 2018
Original languageEnglish
Awarding Institution
  • The University of Manchester
SupervisorStephen Williams (Supervisor) & Laura Parkes (Supervisor)


  • J-editing
  • GABA
  • Glutamate
  • Glutathione
  • spectroscopy
  • Glutamine

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