Metabolomic Profiling in Inflammatory Bowel Disease

Abstract

AbstractThe University of Manchester Colette JohnstonDegree of Doctor of MedicineMetabolomics Profiling in Inflammatory Bowel Disease13th Feb 2014IntroductionInflammatory bowel disease is a common, complex relapsing disorder characterised by immune dysregulation, altered intestinal permeability and microbial insult. Limited knowledge is available regarding the metabolic changes observed during progression of the disease, and limited biomarkers of disease available that have been validated and shown to be of sound clinical value.Aim of StudyA two stage metabolomics approach was adopted to determine if metabolic signature profiles, could distinguish inflammatory bowel disease Crohn's disease (CD) patients from ulcerative colitis (UC) patients and from healthy controls.MethodsA serum metabolomics approach was undertaken to define metabolic changes associated with UC and CD. Serum samples from a discovery study of 30 UC, 30 CD and 29 ethnically, age and gender matched controls were analysed by ultra-performance liquid chromatography mass spectrometry. A subsequent validation study was preformed using 28UC, 31CD, and 29 gender matched controls were also analysed using UPLC-MS.ResultsClasses of metabolites, identified as biologically interesting and at significantly different levels (p

Date of Award	1 Aug 2014
Original language	English
Awarding Institution	The University of Manchester
Supervisor	William Newman (Supervisor)